WEKO3
Item
Establishment ofanovelgeneexpressionmethod,BICES (biomass-induciblechromosome-basedexpressionsystem),and its application to the production of 2,3-butanediol and acetoin.
https://aist.repo.nii.ac.jp/records/2002031
https://aist.repo.nii.ac.jp/records/200203105f33124-5917-4a34-8700-67f3f7115ab2
| Item type | Research Data (v9)(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PubDate | 2014-01-01 | |||||||||||
| Data name | ||||||||||||
| Title | Establishment ofanovelgeneexpressionmethod,BICES (biomass-induciblechromosome-basedexpressionsystem),and its application to the production of 2,3-butanediol and acetoin. | |||||||||||
| Language | en | |||||||||||
| Description of data | ||||||||||||
| Description Type | Abstract | |||||||||||
| Description | In this study, we describe a novel method for producing valuable chemicals from glucose and xylose in Escherichia coli. The notable features in our method are avoidance of plasmids and expensive inducers for foreign gene expression to reduce production coss; foreign genes are knocked into the chromosome, and their expression is induced with xylose that is present in most biomass feedstock. As loci for the gene knock-in, lacZYA and some pseudogenes are chosen to minimize unexpected effects of the knock-in on cell physiology. The promoter of xylF is inducible with xylose and is combined with the T7RNApolymerase–T7 promoter system to ensure strong gene expression. This expression system was named BICES (biomass-induciblechromosome-basedexpressionsystem). As examples of BICES application, 2,3-butanediol and acetoin were successfully produced from glucose and xylose, and the maximal concentrations reached 54gL1 [99.6% in(R,S)-form] and31gL1, respectively. 2,3-Butanediol and acetoin are industrially important chemicals that are, at present, produced primarily through petro-chemical processes. To demonstrate us ability of BICES in practical situations, we produced these chemicals from a saccharified cedar solution. From these results, we can conclude that BICES is suitable for practical production of valuable chemicals from biomass. | |||||||||||
| Language | en | |||||||||||
| Author (Creator) name |
中島 信孝
× 中島 信孝
× 秋田 紘長
× 星野 保
|
|||||||||||
| Access Rights | ||||||||||||
| Access Rights | open access | |||||||||||
| Access Rights URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| APC | ||||||||||||
| APC | Not required | |||||||||||
| Rights Holder | ||||||||||||
| Right Holder Name | 中島 信孝 | |||||||||||
| Language | en | |||||||||||
| Publisher | ||||||||||||
| Publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | |||||||||||
| Language | en | |||||||||||
| Date | ||||||||||||
| Date | 2014-01-01 | |||||||||||
| Date Type | Issued | |||||||||||
| Language | ||||||||||||
| Language | eng | |||||||||||
| Resource Type | ||||||||||||
| Resource Type Identifier | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| Resource Type | journal article | |||||||||||
| Identifier | ||||||||||||
| Identifier | 10.1016/j.ymben.2014.07.011 | |||||||||||
| Identifier Type | DOI | |||||||||||
| Relation | ||||||||||||
| Relation Type | isVersionOf | |||||||||||
| Identifier Type | URI | |||||||||||
| Related Identifier | http://www.sciencedirect.com/science/article/pii/S1096717614001025 | |||||||||||
| Language | ja | |||||||||||
| Related Title | 関連 URI | |||||||||||
| Source Title | ||||||||||||
| Source Title | METABOLIC ENGINEERING | |||||||||||
| Language | en | |||||||||||
| Volume Number | ||||||||||||
| Volume | 25 | |||||||||||
| Page Start | ||||||||||||
| Start Page | 204 | |||||||||||
| Page End | ||||||||||||
| End Page | 214 | |||||||||||
