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The effect of enzymatically crosslinked tilapia scale collagen for osteoblastic differentiation of human mesenchymal stem cells
https://aist.repo.nii.ac.jp/records/2001974
https://aist.repo.nii.ac.jp/records/2001974ac368c63-ce25-49cd-a734-39f40bd843e7
| Item type | Research Data (v9)(1) | |||||||||||||||
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| PubDate | 2015-01-01 | |||||||||||||||
| Data name | ||||||||||||||||
| Title | The effect of enzymatically crosslinked tilapia scale collagen for osteoblastic differentiation of human mesenchymal stem cells | |||||||||||||||
| Language | en | |||||||||||||||
| Description of data | ||||||||||||||||
| Description Type | Abstract | |||||||||||||||
| Description | テラピアコラーゲンの持つ間葉系幹細胞の骨芽細胞への分化誘導効果を、トランスグルタミナーゼによる架橋をコラーゲンに施すことにより、長期間持続できることが分かった。 Abstract: In this study, fish collagen derived from tilapia scales which are free from the risk of infection was employed to fabricate a 3D porous scaffold. Tilapia scale collagen (TSC) scaffolds were modified using microbial transglutaminase (mTGase) enzyme as a catalyst to preserve the inherent properties of collagen material. Human mesenchymal stem cells (hMSCs) were incubated with various substitutes including TSC and porcine collagen-coated cell culture dishes with or without mTGase, TSC and porcine collagen scaffolds crosslinked by mTGase. Differentiation of hMSCs was verified using alkaline phosphatase activity and osteocalcin assays. A remarkably enhanced expression of osteoblastic differentiation was observed selectively with TSC-coated dishes with/without mTGase and TSC collagen scaffold crosslinked by mTGase. Thus, the combination of TSC and the mTGase crosslinking method will be useful for osteoblastic differentiation and bone tissue engineering. | |||||||||||||||
| Language | en | |||||||||||||||
| Author (Creator) name |
Hwanhee Oh
× Hwanhee Oh
× 植村 壽公
× Isamu Yamaguchi
× Toshiyuki Ikoma
× Junzo Tanaka
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| Access Rights | ||||||||||||||||
| Access Rights | open access | |||||||||||||||
| Access Rights URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||
| APC | ||||||||||||||||
| APC | Not required | |||||||||||||||
| Rights Holder | ||||||||||||||||
| Right Holder Name | Hwanhee Oh | |||||||||||||||
| Language | en | |||||||||||||||
| Publisher | ||||||||||||||||
| Publisher | SAGE PUBLICATIONS LTD | |||||||||||||||
| Language | en | |||||||||||||||
| Date | ||||||||||||||||
| Date | 2015-01-01 | |||||||||||||||
| Date Type | Issued | |||||||||||||||
| Language | ||||||||||||||||
| Language | eng | |||||||||||||||
| Resource Type | ||||||||||||||||
| Resource Type Identifier | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| Resource Type | journal article | |||||||||||||||
| Identifier | ||||||||||||||||
| Identifier | 10.1177/0883911515595240 | |||||||||||||||
| Identifier Type | DOI | |||||||||||||||
| Relation | ||||||||||||||||
| Relation Type | isVersionOf | |||||||||||||||
| Identifier Type | URI | |||||||||||||||
| Related Identifier | http://journals.sagepub.com/doi/abs/10.1177/0883911515595240 | |||||||||||||||
| Language | ja | |||||||||||||||
| Related Title | 関連 URI | |||||||||||||||
| Source Title | ||||||||||||||||
| Source Title | JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS | |||||||||||||||
| Language | en | |||||||||||||||
| Volume Number | ||||||||||||||||
| Volume | on line published | |||||||||||||||
