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EFFECT OF THE TRANSCRANIAL DIRECT CURRENT STIMULATION ON PERMEABILITY OF BLOOD BRAIN BARRIER IN THE RAT
https://aist.repo.nii.ac.jp/records/2001878
https://aist.repo.nii.ac.jp/records/200187842bab195-e23a-4955-85e9-9a463db1ac36
| Item type | Research Data (v9)(1) | |||||||||||||
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| PubDate | 2015-01-01 | |||||||||||||
| Data name | ||||||||||||||
| Title | EFFECT OF THE TRANSCRANIAL DIRECT CURRENT STIMULATION ON PERMEABILITY OF BLOOD BRAIN BARRIER IN THE RAT | |||||||||||||
| Language | en | |||||||||||||
| Description of data | ||||||||||||||
| Description Type | Abstract | |||||||||||||
| Description | Background: The blood-brain barrier (BBB) is a major hurdle for successful CNS drug development. Many high potential “would-be” CNS drug (especially biopharmaceuticals) can currently not be made available to the brain because they do not (adequately) cross the blood-brain barrier. Direct electric stimulation is known to influence the brain cells activities but effects on BBB permeability remain to be shown. Hypothesis: Hypothesizing that noninvasive brain stimulation approach so called transcranial direct current stimulation (tDCS) might disrupt the BBB structure, we here studied the effects of tDCS on the rat BBB permeability. Methods: In a randomized crossover design 3 groups of Wistar rat received single stimuli of anodal, cathodal and sham with 500 μA current applied for 30 min under an anesthetized condition. The BBB permeability was judged by Evans Blue (EB) dye extravasation and followed evaluation of the expression and localization of tight junction protein (Claudin-5; CLN5) and endothelial cells (CD31). Results: At 500 μA anodal and sham tDCS exhibited EB extravasation was 1.59 (μg/g tissue) and 1.76 (μg/g tissue) respectively. In contrast, cathodal tDCS leaked 12.51 μg/g of EB dye in brain tissue, likewise depending on the current intensity applied. In addition, cathodal tDCS led to decrease of CLN5 expression compared to anodal and sham tDCS. Whereas, no difference was detected for CD31 stain in anodal-, sham- and cathodal-stimulated groups.Conclusions: The cathodal stimulation significantly increased Evans blue extravasation and decreased signal intensity of the tight junctions in the brain. More importantly, these results demonstrate that cathodal tDCS seems to be a feasible method to facilitate drug delivery for brain diseases. | |||||||||||||
| Language | en | |||||||||||||
| Author (Creator) name |
Nik Mohd Afizan Nik Abd. Rahman
× Nik Mohd Afizan Nik Abd. Rahman
× 渡辺 由美子
× 肥後 範行
× 高島 一郎
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| Access Rights | ||||||||||||||
| Access Rights | open access | |||||||||||||
| Access Rights URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||
| APC | ||||||||||||||
| APC | Not required | |||||||||||||
| Rights Holder | ||||||||||||||
| Right Holder Name | Nik Mohd Afizan Nik Abd. Rahman | |||||||||||||
| Language | en | |||||||||||||
| Publisher | ||||||||||||||
| Publisher | ELSEVIER SCIENCE INC | |||||||||||||
| Language | en | |||||||||||||
| Date | ||||||||||||||
| Date | 2015-01-01 | |||||||||||||
| Date Type | Issued | |||||||||||||
| Language | ||||||||||||||
| Language | eng | |||||||||||||
| Resource Type | ||||||||||||||
| Resource Type Identifier | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| Resource Type | journal article | |||||||||||||
| Identifier | ||||||||||||||
| Identifier | 10.1016/j.brs.2015.01.330 | |||||||||||||
| Identifier Type | DOI | |||||||||||||
| Relation | ||||||||||||||
| Relation Type | isVersionOf | |||||||||||||
| Identifier Type | URI | |||||||||||||
| Related Identifier | http://www.brainstimjrnl.com/article/S1935-861X(15)00754-8/abstract | |||||||||||||
| Language | ja | |||||||||||||
| Related Title | 関連 URI | |||||||||||||
| Source Title | ||||||||||||||
| Source Title | BRAIN STIMULATION | |||||||||||||
| Language | en | |||||||||||||
| Volume Number | ||||||||||||||
| Volume | 8 | |||||||||||||
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| Issue | 2 | |||||||||||||
| Page Start | ||||||||||||||
| Start Page | 417 | |||||||||||||
