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Aminolevulinate synthase 2 mediates erythrocyte differentiation by regulating larval globin expression during Xenopus primary hematopoiesis
https://aist.repo.nii.ac.jp/records/2001842
https://aist.repo.nii.ac.jp/records/20018424c46f459-8ef7-4775-8077-922ae7463a15
| Item type | Research Data (v9)(1) | |||||||||||
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| PubDate | 2015-01-01 | |||||||||||
| Data name | ||||||||||||
| Title | Aminolevulinate synthase 2 mediates erythrocyte differentiation by regulating larval globin expression during Xenopus primary hematopoiesis | |||||||||||
| Language | en | |||||||||||
| Description of data | ||||||||||||
| Description Type | Abstract | |||||||||||
| Description | Hemoglobin synthesis by erythrocytes continues throughout a vertebrate’s lifetime. The mechanism of mammalian heme synthesis has been studied for many years; aminolevulinate synthase 2 (ALAS2), a heme synthetase, is associated with X-linked dominant protoporphyria in humans. Amphibian and mammalian blood cells differ, but little is known about amphibian embryonic hemoglobin synthesis. We investigated the function of the Xenopus alas2 gene (Xalas2) in primitive amphibian erythrocytes and found that it is first expressed in primitive erythroid cells before hemoglobin alpha 3 subunit (hba3) during primary hematopoiesis and in the posterior ventral blood islands at the tailbud stage. Xalas2 is not expressed during secondary hematopoiesis in the dorsal lateral plate. Hemoglobin was barely detectable by o-dianisidine staining and hba3 transcript levels decreased in Xalas2-knockdown embryos. These results suggest that Xalas2 might be able to synthesize hemoglobin during hematopoiesis and mediate erythrocyte differentiation by regulating hba3 expression in Xenopus laevis. | |||||||||||
| Language | en | |||||||||||
| Author (Creator) name |
小川 朝子
× 小川 朝子
× 栗崎 晃
× 伊藤 弓弦
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| Access Rights | ||||||||||||
| Access Rights | open access | |||||||||||
| Access Rights URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| APC | ||||||||||||
| APC | Not required | |||||||||||
| Rights Holder | ||||||||||||
| Right Holder Name | 小川 朝子 | |||||||||||
| Language | en | |||||||||||
| Publisher | ||||||||||||
| Publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | |||||||||||
| Language | en | |||||||||||
| Date | ||||||||||||
| Date | 2015-01-01 | |||||||||||
| Date Type | Issued | |||||||||||
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| Language | eng | |||||||||||
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| Resource Type Identifier | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| Resource Type | journal article | |||||||||||
| Identifier | ||||||||||||
| Identifier | 10.1016/j.bbrc.2014.11.110 | |||||||||||
| Identifier Type | DOI | |||||||||||
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| Relation Type | isVersionOf | |||||||||||
| Identifier Type | URI | |||||||||||
| Related Identifier | http://www.sciencedirect.com/science/article/pii/S0006291X14021512 | |||||||||||
| Language | ja | |||||||||||
| Related Title | 関連 URI | |||||||||||
| Source Title | ||||||||||||
| Source Title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | |||||||||||
| Language | en | |||||||||||
| Volume Number | ||||||||||||
| Volume | 456 | |||||||||||
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| Issue | 1 | |||||||||||
| Page Start | ||||||||||||
| Start Page | 476 | |||||||||||
| Page End | ||||||||||||
| End Page | 481 | |||||||||||
