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Item
Crystal structure of an acetylesterase from Talaromyces cellulolyticus and the importance of a disulfide bond near the active site.
https://aist.repo.nii.ac.jp/records/2001837
https://aist.repo.nii.ac.jp/records/20018371f2d22a9-7455-49ad-8dad-7e3992e6372b
| Item type | Research Data (v9)(1) | |||||||||||||
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| PubDate | 2015-01-01 | |||||||||||||
| Data name | ||||||||||||||
| Title | Crystal structure of an acetylesterase from Talaromyces cellulolyticus and the importance of a disulfide bond near the active site. | |||||||||||||
| Language | en | |||||||||||||
| Description of data | ||||||||||||||
| Description Type | Abstract | |||||||||||||
| Description | Carbohydrate esterase catalyzes the de-O or de-N-acylation of substituted saccharides in plant cell walls and thus has great potential for industrial biomass saccharification. We recently identified the putative carbohydrate esterase family 3 (CE3) from Talaromyces cellulolyticus. Here, we prepared the recombinant catalytic domain of the enzyme and crystallized it. The crystal structure was determined to 1.5Å resolution. From the structural analysis, it was elucidated that a n-octyl-β-d-glucopyranoside bound to near the catalytic triad (Ser10, Asp179 and His182) and was buried in the active site cavity. Site-directed mutagenesis showed that the N-terminal disulfide bond located near the catalytic triad is involved in the activity and structural stability of the enzyme. | |||||||||||||
| Language | en | |||||||||||||
| Author (Creator) name |
渡邊 真宏
× 渡邊 真宏
× 深田はるみ
× 井上 宏之
× 石川 一彦
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| Access Rights | ||||||||||||||
| Access Rights | open access | |||||||||||||
| Access Rights URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||
| APC | ||||||||||||||
| APC | Not required | |||||||||||||
| Rights Holder | ||||||||||||||
| Right Holder Name | 渡邊 真宏 | |||||||||||||
| Language | en | |||||||||||||
| Publisher | ||||||||||||||
| Publisher | ELSEVIER SCIENCE BV | |||||||||||||
| Language | en | |||||||||||||
| Date | ||||||||||||||
| Date | 2015-01-01 | |||||||||||||
| Date Type | Issued | |||||||||||||
| Language | ||||||||||||||
| Language | eng | |||||||||||||
| Resource Type | ||||||||||||||
| Resource Type Identifier | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| Resource Type | journal article | |||||||||||||
| Identifier | ||||||||||||||
| Identifier | 10.1016/j.febslet.2015.03.020 | |||||||||||||
| Identifier Type | DOI | |||||||||||||
| Relation | ||||||||||||||
| Relation Type | isVersionOf | |||||||||||||
| Identifier Type | URI | |||||||||||||
| Related Identifier | http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2015.03.020/abstract | |||||||||||||
| Language | ja | |||||||||||||
| Related Title | 関連 URI | |||||||||||||
| Source Title | ||||||||||||||
| Source Title | FEBS LETTERS | |||||||||||||
| Language | en | |||||||||||||
| Volume Number | ||||||||||||||
| Volume | 589 | |||||||||||||
| Issue Number | ||||||||||||||
| Issue | 11 | |||||||||||||
| Page Start | ||||||||||||||
| Start Page | 1200 | |||||||||||||
| Page End | ||||||||||||||
| End Page | 1206 | |||||||||||||
